ON PHOSPHOTUNGSTIC ACID STAINING IV. SELECTIVITY OF THE STAINING REACTION
نویسندگان
چکیده
منابع مشابه
The Reactivity and Staining of Tissue Proteins with Phosphotungstic Acid
After aldehyde-fixation, treatment with phosphotungstic acid (PTA) in aqueous acidic medium was shown to produce an intense electron-opaque stain with minimal distortion of organelles. Mitochondrial matrix, cisternae of the endoplasmic reticulum, and the Z-band of muscle were densely stained, whereas membranes stood out in negative contrast. Staining of glycogen or lipid was not apparent. Under...
متن کاملThe Reactivity and Staining of Tissue Proteins with Phosphotungstic Acid Lloyd
After aldehyde-fixation, treatment with phosphotungstic acid (PTA) in aqueous acidic medium was shown to produce an intense electron-opaque stain with minimal distortion of organelles. Mitochondrial matrix, cisternae of the endoplasmic reticulum, and the Z-band of muscle were densely stained, whereas membranes stood out in negative contrast. Staining of glycogen or lipid was not apparent. Under...
متن کاملSelective Demonstration of a Type of Synaptic Vesicle by Phosphotungstic Acid Staining
Fine structural analyses of nerve terminals in both the central and in the peripheral nervous systems have demonstrated the presence of synaptic vesicles, which were postulated to be the storage sites of neurotransmitters . Several different types of synaptic vesicles have been described (see ref . 6) . Electron-lucent vesices, 400-500 A in diameter, constitute the most commonly occurring type ...
متن کاملCamillo Golgi's Black Reaction for Staining Neurons
In 1873 Italy, Camillo Golgi created the black reaction technique, which enabled scientists to stain and view the structure of neurons, the specialized cells that compose the nervous system. During the nineteenth century, scientists were studying cells and proposed cell theory, which describes the basic characteristics of cells as fundamental units of life. However, cell theory struggled to exp...
متن کاملStudies on Vital Staining
When large amounts of brilliant vital red are injected into the blood stream of dogs, the dye is gradually removed from circulation, and most of it is deposited in numerous phagocytic cells which are scattered throughout various organs and tissues. The dye occurs largely in the form of tiny red granules crowded together in the cytoplasm of these cells. If Niagara sky blue, a closely related dye...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Histochemistry & Cytochemistry
سال: 1973
ISSN: 0022-1554,1551-5044
DOI: 10.1177/21.2.155